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mouse anti igf 1r  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mouse anti igf 1r
    Mouse Anti Igf 1r, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 533 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/igf+ir/IGF-IR+Antibody/pm41934916-54-46-48
    Average 94 stars, based on 533 article reviews
    mouse anti igf 1r - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Transfection:

    Article Title: Circulating myomiRNAs as biomarkers in patients with Cushing’s syndrome
    Article Snippet: .. After 48 h, on day 4, differentiated C2C12, as well as differentiated C2C12 transfected with miRNA inhibitor, were treated with a single dose of HC for 12 h. The cells were incubated with primary antibodies against Atrogin-1 (12,866-AP Proteintech, Chicago USA,) dilution used 1:50; MuRF-1 (E-AB-60840 Elabscience, Houston, Texas,) dilution used 1:1000, and IGF-IR (sc271606, Santa Cruz Biotechnology Inc.,) dilution used 1:250 in blocking buffer for 2 h at RT. .. Cells were washed thrice in 0.1% Triton/PBS 1X and incubated 1 h with TRIC- or FITC-conjugated secondary antibodies (ImmunoReagents, Inc., Raleigh, NC) diluted 1:500 in blocking buffer; while to detect the nuclei a 4.6-Diamidino-2-phenylindole (DAPI) (Lonza Group Ltd, Basel, Switzerland) staining, diluted in PBS 1X 1:60,000, was used.

    Article Title: Circulating myomiRNAs as biomarkers in patients with Cushing's syndrome.
    Article Snippet: .. After 48 h, on day 4, differentiated C2C12, as well as differentiated C2C12 transfected with miRNA inhibitor, were treated with a single dose of HC for 12 h. The cells were incubated with primary antibodies against Atrogin-1 (12,866-AP Proteintech, Chicago USA,) dilution used 1:50; MuRF-1 (E-AB-60840 Elabscience, Houston, Texas,) dilution used 1:1000, and IGF-IR (sc271606, Santa Cruz Biotechnology Inc.,) dilution used 1:250 in blocking buffer for 2 h at RT. .. Cells were washed thrice in 0.1% Triton/PBS 1X and incubated 1 h with TRICor FITC-conjugated secondary antibodies (ImmunoReagents, Inc., Raleigh, NC) diluted 1:500 in blocking buffer; while to detect the nuclei a 4.6-Diamidino-2-phenylindole (DAPI) (Lonza Group Ltd, Basel, Switzerland) staining, diluted in PBS 1X 1:60,000, was used.

    Article Title: IPF Pathogenesis is Dependent upon TGFβ Induction of IGF-1
    Article Snippet: A summary of pharmacological inhibitors and growth factor treatments found to be optimal in fibroblasts is provided in , unless otherwise noted in a specific method. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Pharmacological Inhibitors Inhibitor Target Catalogue # Source Working Concentration(s) Vehicle SB431542 TGFβ Type 1 Receptor S1067 Selleck Chemicals (Houston, TX, USA) 10 μM 0.1% DMSO LY-294,002 PI3K S1105 Selleck Chemicals (Houston, TX, USA) 20 μM 0.1% DMSO MK2206 AKT S1078 Selleck Chemicals (Houston, TX, USA) 300 nM 0.1% DMSO U0127 MEK V1121 Promega (Madison, WI, USA) 10 μM 0.1% DMSO Rapamycin mTORC1/2 S1039 Selleck Chemicals (Houston, TX, USA) 10 nM 0.1% DMSO OSI-906 IGF-1 Receptor S1091 Selleck Chemicals (Houston, TX, USA) 1 nM, 1 μM, 10 μM 0.1% DMSO Growth Factor Treatments Growth Factor Catalogue # Source Working Concentration(s) Vehicle TGFβ 240-B R&D Systems (Minneapolis, MN, USA) 10 ng/ml 4 mM HCl, 0.1% BSA IGF-1 (mouse) 9897 Cell Signaling (Danvers, MA, USA) 0.2 ng/ml 20mM citrate, pH 3.0 IGF-1 (human) ab9573 Abcam (Cambridge, MA, USA) 2 ng/ml dH 2 O Open in a separate window Summary of cell culture treatments. .. For siRNA knockdown of IGF-1R in AKR-2B murine fibroblasts or MRC5 human lung fibroblasts, cells were transiently transfected with 40 nM of small interfering RNA (siRNA) to IGF-IR (sc-35638, mouse; sc-29358, human; Santa Cruz Biotechnology Santa Cruz, CA, USA) or non-targeting control (NT, sc-37007; Santa Cruz) according to the manufacturer’s protocol. ..

    Incubation:

    Article Title: Circulating myomiRNAs as biomarkers in patients with Cushing’s syndrome
    Article Snippet: .. After 48 h, on day 4, differentiated C2C12, as well as differentiated C2C12 transfected with miRNA inhibitor, were treated with a single dose of HC for 12 h. The cells were incubated with primary antibodies against Atrogin-1 (12,866-AP Proteintech, Chicago USA,) dilution used 1:50; MuRF-1 (E-AB-60840 Elabscience, Houston, Texas,) dilution used 1:1000, and IGF-IR (sc271606, Santa Cruz Biotechnology Inc.,) dilution used 1:250 in blocking buffer for 2 h at RT. .. Cells were washed thrice in 0.1% Triton/PBS 1X and incubated 1 h with TRIC- or FITC-conjugated secondary antibodies (ImmunoReagents, Inc., Raleigh, NC) diluted 1:500 in blocking buffer; while to detect the nuclei a 4.6-Diamidino-2-phenylindole (DAPI) (Lonza Group Ltd, Basel, Switzerland) staining, diluted in PBS 1X 1:60,000, was used.

    Article Title: Circulating myomiRNAs as biomarkers in patients with Cushing's syndrome.
    Article Snippet: .. After 48 h, on day 4, differentiated C2C12, as well as differentiated C2C12 transfected with miRNA inhibitor, were treated with a single dose of HC for 12 h. The cells were incubated with primary antibodies against Atrogin-1 (12,866-AP Proteintech, Chicago USA,) dilution used 1:50; MuRF-1 (E-AB-60840 Elabscience, Houston, Texas,) dilution used 1:1000, and IGF-IR (sc271606, Santa Cruz Biotechnology Inc.,) dilution used 1:250 in blocking buffer for 2 h at RT. .. Cells were washed thrice in 0.1% Triton/PBS 1X and incubated 1 h with TRICor FITC-conjugated secondary antibodies (ImmunoReagents, Inc., Raleigh, NC) diluted 1:500 in blocking buffer; while to detect the nuclei a 4.6-Diamidino-2-phenylindole (DAPI) (Lonza Group Ltd, Basel, Switzerland) staining, diluted in PBS 1X 1:60,000, was used.

    Article Title: Improvement of oocyte competence and in vitro oocyte maturation with EGF and IGF-I in Guinea pig model.
    Article Snippet: Briefly, endogenous peroxidase activity was blocked in deparaffinized sections with normal goat serum (1:10, Santa Cruz Biotechnology). .. Rabbit polyclonal IgG EGF-R and IGF-IR (both 1:100, Santa Cruz Biotechnology) antibodies were incubated overnight at 4 ◦C, whereas for negative control sections PBS was used instead of primary antibody. .. Subsequently, sections were incubated with a secondary biotinylated anti-rabbit IgG antibody (1:200, Vector Laboratories) and then ABC (Vector Elite kit, Vector Laboratories) was used.

    Blocking Assay:

    Article Title: Circulating myomiRNAs as biomarkers in patients with Cushing’s syndrome
    Article Snippet: .. After 48 h, on day 4, differentiated C2C12, as well as differentiated C2C12 transfected with miRNA inhibitor, were treated with a single dose of HC for 12 h. The cells were incubated with primary antibodies against Atrogin-1 (12,866-AP Proteintech, Chicago USA,) dilution used 1:50; MuRF-1 (E-AB-60840 Elabscience, Houston, Texas,) dilution used 1:1000, and IGF-IR (sc271606, Santa Cruz Biotechnology Inc.,) dilution used 1:250 in blocking buffer for 2 h at RT. .. Cells were washed thrice in 0.1% Triton/PBS 1X and incubated 1 h with TRIC- or FITC-conjugated secondary antibodies (ImmunoReagents, Inc., Raleigh, NC) diluted 1:500 in blocking buffer; while to detect the nuclei a 4.6-Diamidino-2-phenylindole (DAPI) (Lonza Group Ltd, Basel, Switzerland) staining, diluted in PBS 1X 1:60,000, was used.

    Article Title: Circulating myomiRNAs as biomarkers in patients with Cushing's syndrome.
    Article Snippet: .. After 48 h, on day 4, differentiated C2C12, as well as differentiated C2C12 transfected with miRNA inhibitor, were treated with a single dose of HC for 12 h. The cells were incubated with primary antibodies against Atrogin-1 (12,866-AP Proteintech, Chicago USA,) dilution used 1:50; MuRF-1 (E-AB-60840 Elabscience, Houston, Texas,) dilution used 1:1000, and IGF-IR (sc271606, Santa Cruz Biotechnology Inc.,) dilution used 1:250 in blocking buffer for 2 h at RT. .. Cells were washed thrice in 0.1% Triton/PBS 1X and incubated 1 h with TRICor FITC-conjugated secondary antibodies (ImmunoReagents, Inc., Raleigh, NC) diluted 1:500 in blocking buffer; while to detect the nuclei a 4.6-Diamidino-2-phenylindole (DAPI) (Lonza Group Ltd, Basel, Switzerland) staining, diluted in PBS 1X 1:60,000, was used.

    Immunostaining:

    Article Title: The Effects of Juglone on Cell Proliferation and Insulin-Like Growth Factor I Receptor/Phosphoinositide 3-Kinase/p85 (IGF-IR/PI3K/p85) Signaling Pathway in Pancreatic Cancer
    Article Snippet: .. Immunostaining was performed using primary antibodies for IGF-IR (mouse monoclonal, Santa Cruz Biotechnology, sc-271606, RRID: AB_10650003) and PCNA (mouse monoclonal, Santa Cruz Biotechnology, sc-56, RRID: AB_628110), along with fluorescein isothiocyanate (FITC)-labeled secondary antibodies (goat anti-mouse immunoglobulin G [IgG] H&L (Alexa Fluor® 488, Abcam, ab150113, RRID: AB_2576208) and 4′,6-diamidino-2-phenylindole (DAPI) as a nuclear stain. ..

    Staining:

    Article Title: The Effects of Juglone on Cell Proliferation and Insulin-Like Growth Factor I Receptor/Phosphoinositide 3-Kinase/p85 (IGF-IR/PI3K/p85) Signaling Pathway in Pancreatic Cancer
    Article Snippet: .. Immunostaining was performed using primary antibodies for IGF-IR (mouse monoclonal, Santa Cruz Biotechnology, sc-271606, RRID: AB_10650003) and PCNA (mouse monoclonal, Santa Cruz Biotechnology, sc-56, RRID: AB_628110), along with fluorescein isothiocyanate (FITC)-labeled secondary antibodies (goat anti-mouse immunoglobulin G [IgG] H&L (Alexa Fluor® 488, Abcam, ab150113, RRID: AB_2576208) and 4′,6-diamidino-2-phenylindole (DAPI) as a nuclear stain. ..

    Knockdown:

    Article Title: IPF Pathogenesis is Dependent upon TGFβ Induction of IGF-1
    Article Snippet: A summary of pharmacological inhibitors and growth factor treatments found to be optimal in fibroblasts is provided in , unless otherwise noted in a specific method. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Pharmacological Inhibitors Inhibitor Target Catalogue # Source Working Concentration(s) Vehicle SB431542 TGFβ Type 1 Receptor S1067 Selleck Chemicals (Houston, TX, USA) 10 μM 0.1% DMSO LY-294,002 PI3K S1105 Selleck Chemicals (Houston, TX, USA) 20 μM 0.1% DMSO MK2206 AKT S1078 Selleck Chemicals (Houston, TX, USA) 300 nM 0.1% DMSO U0127 MEK V1121 Promega (Madison, WI, USA) 10 μM 0.1% DMSO Rapamycin mTORC1/2 S1039 Selleck Chemicals (Houston, TX, USA) 10 nM 0.1% DMSO OSI-906 IGF-1 Receptor S1091 Selleck Chemicals (Houston, TX, USA) 1 nM, 1 μM, 10 μM 0.1% DMSO Growth Factor Treatments Growth Factor Catalogue # Source Working Concentration(s) Vehicle TGFβ 240-B R&D Systems (Minneapolis, MN, USA) 10 ng/ml 4 mM HCl, 0.1% BSA IGF-1 (mouse) 9897 Cell Signaling (Danvers, MA, USA) 0.2 ng/ml 20mM citrate, pH 3.0 IGF-1 (human) ab9573 Abcam (Cambridge, MA, USA) 2 ng/ml dH 2 O Open in a separate window Summary of cell culture treatments. .. For siRNA knockdown of IGF-1R in AKR-2B murine fibroblasts or MRC5 human lung fibroblasts, cells were transiently transfected with 40 nM of small interfering RNA (siRNA) to IGF-IR (sc-35638, mouse; sc-29358, human; Santa Cruz Biotechnology Santa Cruz, CA, USA) or non-targeting control (NT, sc-37007; Santa Cruz) according to the manufacturer’s protocol. ..

    Small Interfering RNA:

    Article Title: IPF Pathogenesis is Dependent upon TGFβ Induction of IGF-1
    Article Snippet: A summary of pharmacological inhibitors and growth factor treatments found to be optimal in fibroblasts is provided in , unless otherwise noted in a specific method. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Pharmacological Inhibitors Inhibitor Target Catalogue # Source Working Concentration(s) Vehicle SB431542 TGFβ Type 1 Receptor S1067 Selleck Chemicals (Houston, TX, USA) 10 μM 0.1% DMSO LY-294,002 PI3K S1105 Selleck Chemicals (Houston, TX, USA) 20 μM 0.1% DMSO MK2206 AKT S1078 Selleck Chemicals (Houston, TX, USA) 300 nM 0.1% DMSO U0127 MEK V1121 Promega (Madison, WI, USA) 10 μM 0.1% DMSO Rapamycin mTORC1/2 S1039 Selleck Chemicals (Houston, TX, USA) 10 nM 0.1% DMSO OSI-906 IGF-1 Receptor S1091 Selleck Chemicals (Houston, TX, USA) 1 nM, 1 μM, 10 μM 0.1% DMSO Growth Factor Treatments Growth Factor Catalogue # Source Working Concentration(s) Vehicle TGFβ 240-B R&D Systems (Minneapolis, MN, USA) 10 ng/ml 4 mM HCl, 0.1% BSA IGF-1 (mouse) 9897 Cell Signaling (Danvers, MA, USA) 0.2 ng/ml 20mM citrate, pH 3.0 IGF-1 (human) ab9573 Abcam (Cambridge, MA, USA) 2 ng/ml dH 2 O Open in a separate window Summary of cell culture treatments. .. For siRNA knockdown of IGF-1R in AKR-2B murine fibroblasts or MRC5 human lung fibroblasts, cells were transiently transfected with 40 nM of small interfering RNA (siRNA) to IGF-IR (sc-35638, mouse; sc-29358, human; Santa Cruz Biotechnology Santa Cruz, CA, USA) or non-targeting control (NT, sc-37007; Santa Cruz) according to the manufacturer’s protocol. ..

    Control:

    Article Title: IPF Pathogenesis is Dependent upon TGFβ Induction of IGF-1
    Article Snippet: A summary of pharmacological inhibitors and growth factor treatments found to be optimal in fibroblasts is provided in , unless otherwise noted in a specific method. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Pharmacological Inhibitors Inhibitor Target Catalogue # Source Working Concentration(s) Vehicle SB431542 TGFβ Type 1 Receptor S1067 Selleck Chemicals (Houston, TX, USA) 10 μM 0.1% DMSO LY-294,002 PI3K S1105 Selleck Chemicals (Houston, TX, USA) 20 μM 0.1% DMSO MK2206 AKT S1078 Selleck Chemicals (Houston, TX, USA) 300 nM 0.1% DMSO U0127 MEK V1121 Promega (Madison, WI, USA) 10 μM 0.1% DMSO Rapamycin mTORC1/2 S1039 Selleck Chemicals (Houston, TX, USA) 10 nM 0.1% DMSO OSI-906 IGF-1 Receptor S1091 Selleck Chemicals (Houston, TX, USA) 1 nM, 1 μM, 10 μM 0.1% DMSO Growth Factor Treatments Growth Factor Catalogue # Source Working Concentration(s) Vehicle TGFβ 240-B R&D Systems (Minneapolis, MN, USA) 10 ng/ml 4 mM HCl, 0.1% BSA IGF-1 (mouse) 9897 Cell Signaling (Danvers, MA, USA) 0.2 ng/ml 20mM citrate, pH 3.0 IGF-1 (human) ab9573 Abcam (Cambridge, MA, USA) 2 ng/ml dH 2 O Open in a separate window Summary of cell culture treatments. .. For siRNA knockdown of IGF-1R in AKR-2B murine fibroblasts or MRC5 human lung fibroblasts, cells were transiently transfected with 40 nM of small interfering RNA (siRNA) to IGF-IR (sc-35638, mouse; sc-29358, human; Santa Cruz Biotechnology Santa Cruz, CA, USA) or non-targeting control (NT, sc-37007; Santa Cruz) according to the manufacturer’s protocol. ..

    Negative Control:

    Article Title: Improvement of oocyte competence and in vitro oocyte maturation with EGF and IGF-I in Guinea pig model.
    Article Snippet: Briefly, endogenous peroxidase activity was blocked in deparaffinized sections with normal goat serum (1:10, Santa Cruz Biotechnology). .. Rabbit polyclonal IgG EGF-R and IGF-IR (both 1:100, Santa Cruz Biotechnology) antibodies were incubated overnight at 4 ◦C, whereas for negative control sections PBS was used instead of primary antibody. .. Subsequently, sections were incubated with a secondary biotinylated anti-rabbit IgG antibody (1:200, Vector Laboratories) and then ABC (Vector Elite kit, Vector Laboratories) was used.



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    RVG-EVs@Echi attenuate oxidative stress in the in vitro PD models. ( a ) Representative blots and ( b ) quantification of the expression levels of IGF2, P-PI3K, PI3K, P-Akt, Akt, and Nrf2 in MN9D cells. ( c ) Representative blots and ( d ) quantification of Bcl-2, Bax, and the resulting Bcl-2/Bax ratio in MN9D cells. ( e , g ) Representative blots and ( f , h ) quantification showing the effects <t>of</t> <t>the</t> <t>IGF-1R/IR</t> <t>inhibitor</t> <t>NVP-AEW541</t> in MN9D cells. ( i ) Representative graphs of ROS generation in control or MPP+-treated cells upon RVG-EVs@Echi treatment. Scale bars, 50 μm and 10 μm for the original and magnified images, respectively. ( k ) MitoSOX was used to stain live control or MPP+-treated cells upon treatment with RVG-EVs@Echi. Scale bars, 50 μm and 10 μm for the original and magnified images, respectively. ( j , l ) Quantification of the relative ROS and MitoSOX fluorescence intensity. n = 6 per group. ( m ) Representative flow cytometry histograms and ( n ) quantification of mean fluorescence intensity (MFI) showing intracellular ROS levels in MN9D cells. n = 3 per group. Statistical Analysis: For (a-d): Groups are Control, MPP+, and MPP + + RVG-EVs@Echi. n = 3 per group. * p < 0.05, ** p < 0.01 vs. Control group; # p < 0.05, ## p < 0.01 vs. MPP + group. For (e-h): Groups are Control, MPP + + RVG-EVs@Echi, and MPP + + RVG-EVs@Echi + NVP-AEW541. n = 3 per group. * p < 0.05, ** p < 0.01 vs. Control group; # p < 0.05, ## p < 0.01 vs. MPP + + RVG-EVs@Echi group. For (i-n): Groups are Control, RVG-EVs@Echi, MPP+, and MPP + + RVG-EVs@Echi. * p < 0.05, ** p < 0.01 vs. Control group; # p < 0.05, ## p < 0.01 vs. RVG-EVs@Echi group; & p < 0.05, && p < 0.01 vs. MPP + group
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    RVG-EVs@Echi attenuate oxidative stress in the in vitro PD models. ( a ) Representative blots and ( b ) quantification of the expression levels of IGF2, P-PI3K, PI3K, P-Akt, Akt, and Nrf2 in MN9D cells. ( c ) Representative blots and ( d ) quantification of Bcl-2, Bax, and the resulting Bcl-2/Bax ratio in MN9D cells. ( e , g ) Representative blots and ( f , h ) quantification showing the effects <t>of</t> <t>the</t> <t>IGF-1R/IR</t> <t>inhibitor</t> <t>NVP-AEW541</t> in MN9D cells. ( i ) Representative graphs of ROS generation in control or MPP+-treated cells upon RVG-EVs@Echi treatment. Scale bars, 50 μm and 10 μm for the original and magnified images, respectively. ( k ) MitoSOX was used to stain live control or MPP+-treated cells upon treatment with RVG-EVs@Echi. Scale bars, 50 μm and 10 μm for the original and magnified images, respectively. ( j , l ) Quantification of the relative ROS and MitoSOX fluorescence intensity. n = 6 per group. ( m ) Representative flow cytometry histograms and ( n ) quantification of mean fluorescence intensity (MFI) showing intracellular ROS levels in MN9D cells. n = 3 per group. Statistical Analysis: For (a-d): Groups are Control, MPP+, and MPP + + RVG-EVs@Echi. n = 3 per group. * p < 0.05, ** p < 0.01 vs. Control group; # p < 0.05, ## p < 0.01 vs. MPP + group. For (e-h): Groups are Control, MPP + + RVG-EVs@Echi, and MPP + + RVG-EVs@Echi + NVP-AEW541. n = 3 per group. * p < 0.05, ** p < 0.01 vs. Control group; # p < 0.05, ## p < 0.01 vs. MPP + + RVG-EVs@Echi group. For (i-n): Groups are Control, RVG-EVs@Echi, MPP+, and MPP + + RVG-EVs@Echi. * p < 0.05, ** p < 0.01 vs. Control group; # p < 0.05, ## p < 0.01 vs. RVG-EVs@Echi group; & p < 0.05, && p < 0.01 vs. MPP + group
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    Image Search Results


    RVG-EVs@Echi attenuate oxidative stress in the in vitro PD models. ( a ) Representative blots and ( b ) quantification of the expression levels of IGF2, P-PI3K, PI3K, P-Akt, Akt, and Nrf2 in MN9D cells. ( c ) Representative blots and ( d ) quantification of Bcl-2, Bax, and the resulting Bcl-2/Bax ratio in MN9D cells. ( e , g ) Representative blots and ( f , h ) quantification showing the effects of the IGF-1R/IR inhibitor NVP-AEW541 in MN9D cells. ( i ) Representative graphs of ROS generation in control or MPP+-treated cells upon RVG-EVs@Echi treatment. Scale bars, 50 μm and 10 μm for the original and magnified images, respectively. ( k ) MitoSOX was used to stain live control or MPP+-treated cells upon treatment with RVG-EVs@Echi. Scale bars, 50 μm and 10 μm for the original and magnified images, respectively. ( j , l ) Quantification of the relative ROS and MitoSOX fluorescence intensity. n = 6 per group. ( m ) Representative flow cytometry histograms and ( n ) quantification of mean fluorescence intensity (MFI) showing intracellular ROS levels in MN9D cells. n = 3 per group. Statistical Analysis: For (a-d): Groups are Control, MPP+, and MPP + + RVG-EVs@Echi. n = 3 per group. * p < 0.05, ** p < 0.01 vs. Control group; # p < 0.05, ## p < 0.01 vs. MPP + group. For (e-h): Groups are Control, MPP + + RVG-EVs@Echi, and MPP + + RVG-EVs@Echi + NVP-AEW541. n = 3 per group. * p < 0.05, ** p < 0.01 vs. Control group; # p < 0.05, ## p < 0.01 vs. MPP + + RVG-EVs@Echi group. For (i-n): Groups are Control, RVG-EVs@Echi, MPP+, and MPP + + RVG-EVs@Echi. * p < 0.05, ** p < 0.01 vs. Control group; # p < 0.05, ## p < 0.01 vs. RVG-EVs@Echi group; & p < 0.05, && p < 0.01 vs. MPP + group

    Journal: Journal of Nanobiotechnology

    Article Title: RVG-targeted extracellular vesicles loaded with echinatin attenuate dopaminergic neurodegeneration via the IGF-2/PI3K/Akt pathway in Parkinson’s disease mice

    doi: 10.1186/s12951-025-03997-5

    Figure Lengend Snippet: RVG-EVs@Echi attenuate oxidative stress in the in vitro PD models. ( a ) Representative blots and ( b ) quantification of the expression levels of IGF2, P-PI3K, PI3K, P-Akt, Akt, and Nrf2 in MN9D cells. ( c ) Representative blots and ( d ) quantification of Bcl-2, Bax, and the resulting Bcl-2/Bax ratio in MN9D cells. ( e , g ) Representative blots and ( f , h ) quantification showing the effects of the IGF-1R/IR inhibitor NVP-AEW541 in MN9D cells. ( i ) Representative graphs of ROS generation in control or MPP+-treated cells upon RVG-EVs@Echi treatment. Scale bars, 50 μm and 10 μm for the original and magnified images, respectively. ( k ) MitoSOX was used to stain live control or MPP+-treated cells upon treatment with RVG-EVs@Echi. Scale bars, 50 μm and 10 μm for the original and magnified images, respectively. ( j , l ) Quantification of the relative ROS and MitoSOX fluorescence intensity. n = 6 per group. ( m ) Representative flow cytometry histograms and ( n ) quantification of mean fluorescence intensity (MFI) showing intracellular ROS levels in MN9D cells. n = 3 per group. Statistical Analysis: For (a-d): Groups are Control, MPP+, and MPP + + RVG-EVs@Echi. n = 3 per group. * p < 0.05, ** p < 0.01 vs. Control group; # p < 0.05, ## p < 0.01 vs. MPP + group. For (e-h): Groups are Control, MPP + + RVG-EVs@Echi, and MPP + + RVG-EVs@Echi + NVP-AEW541. n = 3 per group. * p < 0.05, ** p < 0.01 vs. Control group; # p < 0.05, ## p < 0.01 vs. MPP + + RVG-EVs@Echi group. For (i-n): Groups are Control, RVG-EVs@Echi, MPP+, and MPP + + RVG-EVs@Echi. * p < 0.05, ** p < 0.01 vs. Control group; # p < 0.05, ## p < 0.01 vs. RVG-EVs@Echi group; & p < 0.05, && p < 0.01 vs. MPP + group

    Article Snippet: To assess the effects of RVG-EVs@Echi, MN9D cells were treated with MPP+ (600 μM) or RVG-EVs@Echi (1 μM) for 24 h. For the IGF-1R inhibition experiment, cells were pre-treated with the IGF-1R/IR inhibitor NVP-AEW541 (0.2 μM; MCE, HY-50866) [ ] for 2 h, followed by co-incubation with MPP + and RVG-EVs@Echi for 24 h.

    Techniques: In Vitro, Expressing, Control, Staining, Fluorescence, Flow Cytometry